Inhibition of NAD⁺ salvage synthesis with FK866 does not affect proliferation or viability of macrophages.

<p>A, B, RAW 264.7 and Maf-KDO proliferation in the presence of different concentrations of FK866, monitored by measuring the increase in protein mass at different intervals over a 24 or 72 hour period. Data represent means of three independent experiments performed in triplicate. C, RAW 264.7 cell viability in the presence of 10 nM FK866. The appearance of the fluorescent apoptosis-specific pSIVA signal was recorded over time and the total pSIVA pixel area was determined per frame using Fiji Imaging software and plotted against time. Lines represent averages from one experiment performed in triplicate. (N.S., not significant; unpaired t-test).