Gastrointestinal stress challenge assays.

<p>Survival of wild type, Δ<i>kpnGH</i> and Δ<i>kpnGH</i>Ω<i>kpnGH</i> in different concentrations of A) Bile (0.2%, 0.5%, 0.75%, 1.0%, and 2.0%), B) Osmotic stress (0.075M, 0.15M, 0.25M, 0.5M, 0.75M, 1M and 2M) and C) Different temperatures (37°C, 42°C and 60°C). Percentage survival was calculated by comparing the number of viable cells in LB medium alone. *, Significant difference (P<0.05, Student t test).