Macrophages-activated MSCs under inflammatory condition promoted the proliferation and migration of HGC-27 cells.

<p>(A) Representative images of cell colony formation assay and histogram of colony number. Data were listed as mean±SD of three wells. *<i>P</i><0.05. (B) Western blot assays for PCNA and VEGF protein levels. (C) Representative images of transwell migration assay and histogram of the number of migrated HGC-27 cells. Magnification, ×100; scale bar = 50 µm. ***<i>P</i><0.001, **<i>P</i><0.01. (D) Western blot assays for E-cadherin, N-cadherin and Vimentin protein levels. (E) Real-time PCR analyses of VEGF, MMP9 and TGF-β mRNA expression. ***<i>P</i><0.001, *<i>P</i><0.05. (F) Western blot assay for Oct4 and Sox2 protein levels in HGC-27 cells. (G) Real-time PCR analyses of Oct4 and Sox2 mRNA expression. **<i>P</i><0.01, *<i>P</i><0.05.