p38α inhibition enhances proliferative capacity of A431 SCC keratinocytes.

<p>(A) The effects of p38α inhibition were assessed by BrdU incorporation in A431 cells transfected with p38α siRNA (p38αKD), or treated with SB203580 (5 µM (SB/5), 10 µM (SB/10) for 24 h. Each histogram represents the results from triplicate cultures; *<i>p</i><0.05. scr, scrambled control siRNA. The levels of cyclin D1 and cdc25C in A431 cells treated with the indicated concentrations of SB203580 for 24 h (B), or following p38α knockdown (C) were assessed by Western blotting (50 µg protein loaded per lane). β-actin was used as an internal loading control.