Pre-diabetic mice have increased body weight, increased insulin levels and decreased glucose tolerance.

<p>A) Body weight B) serum insulin levels, and C) blood glucose during i.p. glucose tolerance test (GTT) were measured in mice fed a low fat (Chow; n = 5) or high fat (n = 4) diet for 15 weeks. Mice were fasted overnight prior to the GTT. Glucose (2 g/kg body weight) was injected i.p. at time 0. D) Area under the curve from GTT test results was calculated for each treatment group. *p<0.001 vs. Chow.