Aberrant <i>myostatin</i> cDNA.

<p>(A) Nine sequenced Blonde d'Aquitaine (BAB) animals carry a 41-bp additional sequence between the exons 2 and 3. (B) Wild-type <i>myostatin</i> cDNA primary structure at the exon2/exon3 junction (WT). (C) The 41-bp extra exon inclusion leads to a premature termination codon. Therefore, translation predicts a truncated protein lacking the entire bioactive region <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0097399#pone.0097399-Wozney1" target="_blank">[2]</a>, encoded by exon 3. The arrow indicates that the extra exon inclusion occurs at the exon2/exon3 junction of the wild-type transcript. Of note, the premature stop codon occurs 29-bp upstream from the exon2/exon3 junction.