AT1 inhibition does not inhibit eNOS or AMPK phosphorylation induced by telmisartan in HUVECs.

<p><b>A</b>. HUVECs were incubated with the vehicle (DMSO) or telmisartan for 2 h under the conditions, in which the AT1R binding pocket was blocked by pretreatment with high concentrations of valsartan (200 µM) for 30 min. Representative immunoblots of eNOS and AMPK are shown. <b>B</b>. Relative phosphorylation levels of eNOS were calculated as the band intensity ratio of phospho-eNOS to total eNOS. Bar graph shows the mean ± SEM of 4 independent experiments. <b>C</b>. Relative phosphorylation levels of AMPK were calculated as the band intensity ratio of phospho-AMPK to total AMPK. Bar graph shows the mean ± SEM of 4 independent experiments. nc, negative control. *, p < 0.05 versus control (nc+DMSO); #, p < 0.05 versus Val+DMSO; †, p < 0.01 versus control; **, p < 0.01 versus Val+DMSO.