The human lung epithelial cell line BEAS-2B is less efficiently infected with Mtb than human DC.

<p>A–B) Primary human DC or the human bronchial epithelial cell line, BEAS-2B, were infected with dsRED Mtb at an MOI of 30, 10, and 3. Fixed cells were assessed for dsRED-H37Rv infection by flow cytometry after 18 hours. FlowJo (TreeStar) was used to calculate the change in geometric mean in BEAS-2B cells or DC for MOI:10. Results are representative of all experiments (N = 3). C–E) DC, BEAS-2B cells, or primary LAEC were infected with Mtb (MOI:7). Infected cells were imaged in an unbiased manner, and the number of bacteria per cell was enumerated. Results are representative of all experiments (N = 3 for DC and BEAS-2B cells, N = 2 for LAEC).