The Mtb vacuole in epithelial cells acquires Class I molecules.

<p>A–C) BEAS-2B cells were infected with dsRED-expressing Mtb or RFP-expressing M. smegmatis at MOI:5 for 4 or 18 hours. Infected cells were fixed and stained for Class I molecules (W6/32). At least 200 intracellular bacteria were counted from 3 independent experiments. Mtb-containing vacuoles were categorized as positive or negative, the mean percent positive and standard error were determined, and a Student's t-test was used to determine statistical significance between groups. A (Mtb); B (Msm). Scale bar = 10 uM. D) Primary LAEC were infected with dsRED-expressing Mtb at MOI:5 for 18 hours and analyzed as described above. Infected cells were fixed and stained with W6/32. Scale bar = 10 uM. Images are representative of Mtb or Msm associated with HLA-I. Error bars represent the mean and SEM for all events.