Nitric oxide production (ng/ml) by J774A.1 macrophages upon treatment with LieIF/IFN-γ at late (A, B) and early (C, D) time points after <i>L. donovani</i> infection.

<p>J774A.1 cells were treated pre-infection (A, C) and post-infection (B, D) with recombinant LieIF (10 µg/ml) and recombinant IFN-γ (1 ng/ml). Nitrite accumulation in the medium was measured by addition of Griess reagent, absorbance reading of the reaction product at 570 nm. All data are presented as the mean ± S.D of at least three independent experiments. Asterisks indicate statistically significant production of NO (p≤0.05).