The <i>Arabidopsis ZIF2</i> gene encodes multiple transcripts.

<p>(A) Exon/intron organization of the <i>ZIF2</i> gene and T-DNA insertion site in the <i>zif2-1</i> mutant. Boxes and lines between boxes denote exons and introns, respectively. The triangle depicts the site of the T-DNA insertion. F1, F1′, F2, F3, R1, R2, and R3 indicate the location of the primers used to detect <i>ZIF2</i> expression in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004375#pgen-1004375-g003" target="_blank">Figures 3A, 3C</a>, <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004375#pgen-1004375-g007" target="_blank">7A, S</a>1 and S4. Scale bar, 200 bp. (B) Structure of the alternative <i>ZIF2</i> transcripts. The coding sequence (CDS) is shown in black and the UTRs in white. Grey boxes indicate portions of the 5′UTR overlapping with the retained intron. RACE1 to RACE6 indicate the location of the gene-specific primers used in the 5′RLM-RACE experiment. Occurrence of each <i>ZIF2</i> transcript was determined according to its detection (number of corresponding 5′RLM-RACE clones) or not (-) by RLM-5′RACE in the indicated tissue. Scale bar, 200 nt.