Probiotics regulate Toll-like receptor (TLR) pathway in the lung 24 hours post CLP.

<p>Reverse transcription and real-time PCR assays were performed to quantify steady-state mRNA levels of TLR-2, TLR-4, MyD88 and NFΚB (p50/p105). (A) TLR-2 and (C) MyD88 were significantly upregulated in the septic group compared to shams (P<0.05) and significantly downregulated in the lungs of LGG and BL treated septic mice compared to untreated septic mice. (B) mRNA levels of TLR-4 remained unchanged in all groups. (D) NFΚB (p50/p105) was significantly upregulated in the septic group compared to shams (P<0.05) and significantly downregulated in the lungs of LGG treated septic mice compared to untreated septic mice. The levels in BL treated mice were decreased but did not reach statistical significance. Shams n = 5 per group; Septic, Septic+LGG, Septic+BL n = 5–7 per group. Data are expressed as the mean ± SE.