Sensitivity to paclitaxel correlates with E2F3 levels in NSCLC.

<p><i>A</i>. cDNA from ten NSCLC cell lines were utilized in real-time PCR to detect endogenous E2F3 expression levels (as compared to GAPDH as a control). The expression levels were then compared to the paclitaxel logIC₅₀ of each line and graphed as shown. <i>B</i>. Lysates from ten NSCLC cell lines were prepared and ran in a Western blot to detect endogenous E2F3 levels. <i>C</i>. Densitometrically analyzed values of the protein expression levels (as compared to β-actin as a control) were then graphed against the paclitaxel logIC₅₀ for each line as shown.