Multiple sequence alignment of MDHs from typical species of Phaeophyceae, Cyanobacteria, Actinobacteria, Proteobacteria, Ascomycota and Codonosigidae.

<p>Identical residues among all MDHs were shown in black boxes. The representative conserved regions among PSLDRs were underlined in black. The deletions of β-sheets in SjM2DH were underlined in green while the extra anti-parallel β-sheet was underlined in red. ▾, residues for substrate-binding; ⧫, residues for NADH-binding. The accession numbers were listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0097935#pone.0097935.s004" target="_blank">File S2</a>.