Influence of TNF-α on GLAST, GLT-1a and GLT-1b mRNAs and proteins in cortical astrocytes from wild-type or hSOD1^G93A rats.

<p>Expression of GLAST (A), GLT-1a (C) and GLT-1b (E) mRNAs as number of copies per microgram of RNA was estimated by RT-qPCR in control conditions or after 72 h exposure to TNF-α (20 ng/mL) using the corresponding cloned cDNA sequences as standards. Data shown are means with SEM conducted from six independent experiments performed in duplicate. Expression of GLAST (B), GLT-1a (D) and GLT-1b (F) proteins was examined in cells maintained in control conditions or treated with TNF-α (20 ng/mL) for 72 h. Immunoblots shown are representative of six independent experiments. Data indicate the levels of the protein of interest normalized to GAPDH and represents means with SEM. *<i>p</i><0.05 for comparison between the same genotype.