Microarray analysis of adipocytes stimulated by <b><i>C. burnetii</i></b>.

<p>Adipocytes differentiated from the fibroblast cell line 3T3-L1 were incubated with 50 bacteria per cell for 8 hours. <b>A</b>, Heatmap representation of modulated genes selected by supervised analysis in stimulated and unstimulated adipocytes. Genes (in rows) and samples (in columns) were organized by hierarchical clustering (Pearson correlation distance, average linkage). Gene expression levels are color coded from blue (down-modulated) to red (up-modulated). <b>B-C</b>, Functional annotation of modulated genes and qRT-PCR results. The modulated genes in the most enriched GO terms are shown (B). qRT-PCR confirmation of some genes of the most enriched GO terms identified by microarray analysis (C). qRT-PCR ratios are presented as bleu bars and microarray ratios are presented as red bars. Both methods gave similar results (Spearman correlation coefficient = 0.85, <i>P</i> value = 0.006).