MEHP exposure affected mRNA and protein expression.

<p>(A) MEHP exposure affected Bax and Bcl-2 mRNA expression levels in HUVEC cells. The HUVEC cells were treated with 0, 6.25, 12.5, 25, 50, and 100 µM MEHP for 24 hours in MEHP treated group. In NAC+MEHP treated group, before MEHP administration the HUVEC cells was pretreated by NAC for 1 hour and then treated with 0 µM and 100 MEHP for 24 hours. The HUVEC cells in control group were only treated with 0.1% dimethyl sulfoxide (DMSO) for 24 hours. Bcl-2 and Bax mRNA expression was quantified after normalization to GAPDH. Data from three independent experiments were presented in the form of mean±SEM; n = 3. * P<0.05 was considered as statistically significant difference compared to the control group. (B,C,D,E,F) MEHP exposure affected cytochrome C, Bax and Bcl-2 protein expression in HUVEC cells. The HUVEC cells were treated with 0, 6.25, 12.5, 25, 50, and 100 µM MEHP for 24 hours in MEHP treated group. In NAC+MEHP treated group, before MEHP administration the HUVEC cells was pretreated by NAC for 1 hours and then treated with 0 µM and 100 MEHP for 24 hours. The HUVEC cells in control group were treated only with 0.1% dimethyl sulfoxide (DMSO) for 24 hours. Experiments were repeated in triplicate.Data presented are the mean±SEM from three independent experiments; n = 6. * P<0.05, statistically significant difference compared with the control group.