Venn diagram of transcripts elevated ≥2-fold in embryos treated or not treated with aphidicolin.

<p>Pronuclear oocytes were cultured in the presence of aphidicholin (Aph) from 6 to 96 hours post activation (hpa), and compared with sibling embryos that were not treated so as to appreciate how many mRNAs are upregulated regardless of the cell cycle progression. (<b>A</b>) Venn diagram showing mRNAs that accumulate after SCNT when the first round of embryonic DNA replication is suppressed (Aph⁺) in comparison to mRNAs that accumulate when the embryo can cycle normally (Aph⁻). (<b>B</b>) Venn diagram showing mRNAs that accumulate after fertilization when the first round of embryonic DNA replication is suppressed (Aph⁺) in comparison to mRNAs that accumulate when the embryo can cycle normally (Aph⁻). In both SCNT and fertilization, mRNAs were considered whose abundance increased at least 2-fold compared to MII oocytes.