<p>(<b>A</b>) Western blot showing the protein levels of HIF-1α in HCT116. Cells were cultured at 20% or 5% O<sub>2</sub> for 72 hours. (<b>B</b>) Western blot showing the protein levels of HIF-1α in normal human keratinocytes cultured for 1 to 4 days at 5% O<sub>2</sub> or treated 500 µM of chemical hypoximimetic CoCl<sub>2</sub> for 16 hours (H). (<b>C</b>) Luciferase assay showing HIF-1α in HCT116 cells. HCT116 were transfected with a PGK-1 luciferase reporter plasmid and a β-galactosidase control plasmid and then cultured for 48 hours at 5% O<sub>2</sub>. β-galactosidase activity was used to normalize luciferase activity. Luciferase activity is expressed as a ratio to 20% O<sub>2</sub> levels. Results show mean values of 3 independent e...
<p>(<b>a</b>) HIF1A gene expression of human MSCs obtained by real-time PCR (n = 6; unpaired t-test)...
<p>A, Western blots showing the time course of HIF-1α and HIF-2α stabilisation and protein levels of...
<p>A) HIF-1α expression at different times (0–24 h) after setup of hypoxia; 3 h+3 h: incubation of c...
<p>(<b>A</b>) Western blot showing the protein levels of HIF-1α in HCT116 HIF<sup>+/+</sup> and HIF<...
<p>A, 143B and MG-63 human OS cells showed increases in HIF-2α protein expression by western blot wh...
<p>(<b>A</b>) Western blot showing the protein levels of HIF-1α, p53, phosphorylated ERK 1/2 (P-MAPK...
<p>In the hypoxic condition, cells were cultured in 0.1% oxygen. The total protein was isolated from...
<div><p>HIF-1 is a transcription factor that mediates the cellular responses to low oxygen environme...
HIF-1 is a transcription factor that mediates the cellular responses to low oxygen environments, mai...
HIF-1 is a transcription factor that mediates the cellular responses to low oxygen environments, mai...
HIF-1 is a transcription factor that mediates the cellular responses to low oxygen environments, mai...
HIF-1 is a transcription factor that mediates the cellular responses to low oxygen environments, mai...
<p>Cells were grown under normoxic conditions (N) or exposed to 1% O<sub>2</sub> for 24 h (H). Inter...
It has been found that hypoxia is an advantageous condition for the culture of pluripotent stem cell...
<p>(A) Schematic drawing of HIF-1 and its regulation in normoxia and hypoxia. The stability of the H...
<p>(<b>a</b>) HIF1A gene expression of human MSCs obtained by real-time PCR (n = 6; unpaired t-test)...
<p>A, Western blots showing the time course of HIF-1α and HIF-2α stabilisation and protein levels of...
<p>A) HIF-1α expression at different times (0–24 h) after setup of hypoxia; 3 h+3 h: incubation of c...
<p>(<b>A</b>) Western blot showing the protein levels of HIF-1α in HCT116 HIF<sup>+/+</sup> and HIF<...
<p>A, 143B and MG-63 human OS cells showed increases in HIF-2α protein expression by western blot wh...
<p>(<b>A</b>) Western blot showing the protein levels of HIF-1α, p53, phosphorylated ERK 1/2 (P-MAPK...
<p>In the hypoxic condition, cells were cultured in 0.1% oxygen. The total protein was isolated from...
<div><p>HIF-1 is a transcription factor that mediates the cellular responses to low oxygen environme...
HIF-1 is a transcription factor that mediates the cellular responses to low oxygen environments, mai...
HIF-1 is a transcription factor that mediates the cellular responses to low oxygen environments, mai...
HIF-1 is a transcription factor that mediates the cellular responses to low oxygen environments, mai...
HIF-1 is a transcription factor that mediates the cellular responses to low oxygen environments, mai...
<p>Cells were grown under normoxic conditions (N) or exposed to 1% O<sub>2</sub> for 24 h (H). Inter...
It has been found that hypoxia is an advantageous condition for the culture of pluripotent stem cell...
<p>(A) Schematic drawing of HIF-1 and its regulation in normoxia and hypoxia. The stability of the H...
<p>(<b>a</b>) HIF1A gene expression of human MSCs obtained by real-time PCR (n = 6; unpaired t-test)...
<p>A, Western blots showing the time course of HIF-1α and HIF-2α stabilisation and protein levels of...
<p>A) HIF-1α expression at different times (0–24 h) after setup of hypoxia; 3 h+3 h: incubation of c...