<p>The reverse transcription in the absence of reverse transcriptase (RTse –) was used to control for amplification from incompletely digested genome DNA. The <i>GAPDH mRNA</i> served as a loading control. The sizes of <i>GPR41</i>, <i>GPR43</i> and <i>GAPDH</i> PCR products were 398 bp, 249 bp and 220 bp, respectively. The bands of the marker were 50 bp, 100 bp, 150 bp, 200 bp, 300 bp, 400 bp, and 500 bp from bottom to above. M: marker; L: liver; S: spleen; I: ileum; C: colon; H: heart; K: kidney; AT: adipose tissue; SM: skeletal muscle.</p
<p>(a) RT-PCR detection of the IgD, Iδ germline, IgM and Iμ germline transcripts in different tissue...
The intestinal mucosal development of piglets (Sus scrofa) during the weaning stage is important to ...
The growth hormone-insulin-like growth factor (GH-IGF) axis is involved in the control of postnatal ...
<p>The vertical axis of the figure was presented as the target genes copies (<i>GPR41</i> and <i>GPR...
<p>The vertical axis of the figure was presented as the target genes copies (<i>GPR41</i> and <i>GPR...
<p><i>β-actin</i> was used as the loading control. M: marker; L: liver; S: spleen; I: ileum; C: colo...
<div><p>Short-chain fatty acids (SCFAs) are not only an important energy source, but they also play ...
Short-chain fatty acids (SCFAs) are not only an important energy source, but they also play a regula...
<p><b>a)</b> The RT-PCR products (1080 bp) of <i>SLA-MIC2</i> exons 2 to 6 in different tissues, amp...
<p>Assessment of transgene expression by RT-PCR. Transgenic pigs: 904, 907, 908, 914, 915, 917, 918,...
Abstract Background Gene expression analysis using real-time RT-PCR (qRT-PCR) is increasingly import...
BackgroundGene expression analysis using real-time RT-PCR (qRT-PCR) is increasingly important in bio...
<p>cDNA was synthesized from 50 ng of RNA and subjected to qPCR using primer/probe sets for Foxp3 an...
<p>Both HNF1α (<b>A</b>) and HNF1β (<b>B</b>) were negative in isolated glomeruli. Liver was used as...
<p><i>GAPDH</i> is used as endogenous reference. Each column represents the mean expression of a tri...
<p>(a) RT-PCR detection of the IgD, Iδ germline, IgM and Iμ germline transcripts in different tissue...
The intestinal mucosal development of piglets (Sus scrofa) during the weaning stage is important to ...
The growth hormone-insulin-like growth factor (GH-IGF) axis is involved in the control of postnatal ...
<p>The vertical axis of the figure was presented as the target genes copies (<i>GPR41</i> and <i>GPR...
<p>The vertical axis of the figure was presented as the target genes copies (<i>GPR41</i> and <i>GPR...
<p><i>β-actin</i> was used as the loading control. M: marker; L: liver; S: spleen; I: ileum; C: colo...
<div><p>Short-chain fatty acids (SCFAs) are not only an important energy source, but they also play ...
Short-chain fatty acids (SCFAs) are not only an important energy source, but they also play a regula...
<p><b>a)</b> The RT-PCR products (1080 bp) of <i>SLA-MIC2</i> exons 2 to 6 in different tissues, amp...
<p>Assessment of transgene expression by RT-PCR. Transgenic pigs: 904, 907, 908, 914, 915, 917, 918,...
Abstract Background Gene expression analysis using real-time RT-PCR (qRT-PCR) is increasingly import...
BackgroundGene expression analysis using real-time RT-PCR (qRT-PCR) is increasingly important in bio...
<p>cDNA was synthesized from 50 ng of RNA and subjected to qPCR using primer/probe sets for Foxp3 an...
<p>Both HNF1α (<b>A</b>) and HNF1β (<b>B</b>) were negative in isolated glomeruli. Liver was used as...
<p><i>GAPDH</i> is used as endogenous reference. Each column represents the mean expression of a tri...
<p>(a) RT-PCR detection of the IgD, Iδ germline, IgM and Iμ germline transcripts in different tissue...
The intestinal mucosal development of piglets (Sus scrofa) during the weaning stage is important to ...
The growth hormone-insulin-like growth factor (GH-IGF) axis is involved in the control of postnatal ...