Immunofluorescence staining of the lumbar spinal cord section of SOD1G93A transgenic mouse transplanted with F3.Olig2-Shh cells.

<p>The grafted F3.Olig2-Shh cells were detected in ventral horn of the spinal cord by immunofluorescence staining with anti-human mitochondria (hMit, green) antibody. hMito-positive F3.Olig2-Shh human cells (green) were doubly positive with neuron-specific markers neurofilament (NF, red) (A∼C) and also with MAP2 (red, D∼F). hMit-NF and hMit-MAP2 double positive motor neurons with large cell bodies were found in the ventral horn of spinal cord. There were no hMit (green)- and GFAP (red)-double positive cells in the spinal cord sections (G∼K), indicating that F3.Olig2-Shh cells did not differentiate into GFAP-positive astrocytes, but into NF-positive or MAP2-positive motor neurons. The hMit (green)-positive F3.Olig2-Shh cells are doubly positive with motor neuron specific markers ChAT (red, J∼L) or Hb9 (red, M∼O) and located in the ventral horn. The white boxes represent magnified areas. The bar indicates 200 µm.