Thermodynamic parameters of the thermal unfolding of Δ10ct-PDZ3 domain and mutants under different acidic pH conditions in 50 mM buffer, obtained from the analysis of DSC experiments^*.

<p>*Experimental conditions were 50 mM acetic/acetate pH 4.0; 50 mM Glycine/HCl pH 2.5–3.5. The error intervals were calculated as described in the text. The values of thermodynamic magnitudes for the U⇆In and I⇆In equilibriums were estimated for a P_ref = 100 µM. The heat capacity functions obtained from the fittings, in kJ·K⁻¹·mol⁻¹ were: <b>pH 4:</b> C_pN = −12.8+(0.105*T); C_pU = −9.3+(0.084*T); C_pIn = −62.4+(0.233*T); C_pI = −35.4+(0.158*T). <b>pH 3.5</b>: C_pN = 2.66+(0.056*T); C_pU = 12.1+(0.034*T); C_pIn = −96.6+(0.348*T); <b>pH 3:</b> C_pN = 1.50+(0.059*T); C_pU = 15.1+(0.026*T); C_pIn = −11.7+(0.105*T); <b>pH 2.5</b>: C_pN = 10.3+(0.032*T); C_pU = 18.1+(0.018*T); C_pIn = −15.3+(0.114*T).</p>a<p>Data obtained from two-state analysis.