Collagen, CD45⁺, CD68⁺, α-SMA and apoptotic cell content and plaque stability scores in <i>SM22α-hDTR ApoE^−/−</i> HFD after sP-selectin treatment for 16 weeks.

<p><b>A)</b> Sections of each aortic segment were analysed for collagen content by immunohistochemistry after 16 weeks of daily s.c injections (22.5 µg/kg/day) of vehicle control (saline matched volume; white bar), sP-selectin (black bar) or sE-selectin (checkered bar). Representative images are given below. Sections of each aortic segment were analysed for <b>B)</b> CD68⁺ (representative images shown below) and <b>C)</b> CD45⁺ cells by immunohistochemistry after 16 weeks of daily s.c injections (22.5 µg/kg/day) of vehicle control (saline matched volume; white bar), sP-selectin (black bar) or sE-selectin (checkered bar) <b>D)</b> αSMA content or <b>E)</b> apoptotic cell content (% TUNEL) by immunohistochemistry after 16 weeks of daily s.c injections (22.5 µg/kg/day) of vehicle control (saline matched volume; white bar), sP-selectin (black bar) or sE-selectin (checkered bar). <b>F)</b> Plaque stability scores were defined as ratio of collagen content over lipid area after 16 weeks of daily s.c injections (22.5 µg/kg/day) of vehicle control (saline matched volume; white bar), sP-selectin (black bar) or sE-selectin (checkered bar). <i>n</i> = 6–9 mice per treatment group. Data represented as Mean (% stain or score) ± SEM where * represents <i>P</i><0.05 (from saline or indicated treatment) as analysed by One-way ANOVA with Tukey’s post-hoc test or unpaired student’s t-test (C, D). Scale bars = 100 µm.