Lasiodin inhibited cell proliferation and migration.

<p>(<b>A</b>), Lasiodin inhibited NPC cell proliferation. The cells treated with the vehicle control (DMSO) were used as the reference group with cell viability set at 100%. (<b>B</b>), The cells were treated with lasiodin at the indicated does for 24 hr. The cellular morphology was observed by the phase contrast microscopy. NPC cells treated with the vehicle control (DMSO) were used as the reference group. (<b>C</b>), The colony formation efficiency was detected by the plate clone formation assay. After incubation for two weeks, the cells were stained with crystal violet staining solution. NPC cells treated with the vehicle control (DMSO) were used as the reference group. (<b>D</b>), Lasiodin mediated the NPC cell migration inhibition. Cell migration was analyzed by the scratch assay. CNE1 and CNE2 were grown to the full confluency. The cell monolayers were wounded with a sterile pipette tip, and washed with the medium to remove the detached cells from the plates. NPC cells were left either untreated or treated with the indicated doses of lasiodin. After 24 hr, the wound gap was observed and photographed. NPC cells treated with the vehicle control (DMSO) were used as the reference group. The figures are representative of three experiments. The data are presented as mean ± S.D. of three separate experiments. * <i>P</i><0.05, the significant differences between treatment and control groups.