Transcript and protein expression levels of cathepsin B (A, C), cathepsin D (B, D) and β-glucorinidase (E, F) in MS and MS9II cells.

<p>Histograms showing decreased levels of C<i>tsb</i> mRNA (A) and <i>Ctsd</i> mRNA (B), but increased levels of <i>Gusb</i> mRNA (E) in MS9II cells compared to MS cells. Immunoblots and respective histograms showed decreased levels of pro-cathepsin B and D but increased levels of mature cathepsins B and D in MS9II cells than in MS cells. Immunoblot analysis of β-glucuronidase level, consistent with mRNA, was enhanced in MS9II cells. The protein levels were normalized to the β-actin and the values from four different experiments are expressed as means ± SEM, *<i>p</i><0.05, **<i>p</i><0.01.