Interactions of GmFDLs with GmFT2a and GmFT5a.

<p>(A) Yeast two-hybridization assays; FD was also included because both GmFT2a and GmFT5a promoted early flowering in Arabidopsis. After cotransformation of the baits and preys, an equal amount of yeast clones were plated on SD-Leu-Trp and SD-Leu-Trp-His-Ade+X-α-gal selective plates, and the plates were incubated at 30°C until the emergence of the yeast clones. (B) BiFC (bimolecular fluorescence complementation) assays to confirm the results of the yeast two-hybridization assays. Arabidopsis protoplasts cotransformed with constructs of FTs or FDs fused to the N-terminal (YN) and C-terminal (YC) halves of YFP, respectively (as indicated), were imaged using a confocal microscope after incubation at room temperature (20°C to 25°C) over 18 hours. Images are shown as YFP, merged YFP and bright field. Scale bars indicate 20 µm.