Hypodiploid cell formation in HeLa cells was inhibited by treatment with CytoD or LatB in the presence of TSA.

<p><b>A:</b> HeLa cells were treated with various concentrations of TSA for 12, 24, or 48<b>B:</b> HeLa cells were treated with 1,000 nM CytoD for 6, 12, or 18 h. <b>C:</b> HeLa cells were treated with 500 nM LatB for 3, 6, or 12 h. <b>D:</b> HeLa cells were treated for 24 h with various concentrations of TSA in the presence or absence of 500 nM LatB or 1,000 nM CytoD. Cells were fixed with 40% ethanol and stained with PI, and then hypodiploid cells were analyzed by flow cytometry. <b>E–F:</b> HeLa cells were treated for 16 h with 1,000 nM TSA in the presence or absence of 500 nM LatB or 1,000 nM CytoD. Cells were stained with annexin V-FITC and PI, and then analyzed by flow cytometry (E). Cells were fixed with 3% paraformaldehyde and stained with DAPI, and then observed under a fluorescence microscope at 400× magnification. Arrows indicate representative cells with chromosome condensation in each group (F, left). The data in the bar graph represent the mean ± SEM. **<i>p</i><0.01 vs. untreated control cells; ^#<i>p</i><0.05 vs. TSA-treated and non-CytoD-treated control cells; ^$<i>p</i><0.05 vs. TSA-treated and non-LatB-treated control cells (F, right).