The inhibitory effect of aptamer BV42 on the first stage of viral attachment to MDCK cell.

<p>BV42 managed to inhibit the attachment of influenza virus (A/Texas/1/77) to MDCK host cells. The attachment of the virus to the host cells was reduced by BV42 aptamer in a dose dependent manner, the IC50 was about 1 nM. To show that the aptamer is affecting the first stage of the infection, the attachment of the virus to the host cells was done in 4°C; in this way the amount of viruses on cells refers to viruses that are attached to cells alone without the effect of the next stages of the viral infection cycle. The amount of the virus attached to the cell culture is expressed as optic density (OD) of the colorimetric product that is produced by enzyme attached to anti-influenza antibody. The inhibitory effect = 100 * [1–OD_(test)/OD_{(no aptamer)}] The error bar indicated standard error (SE).