AdV-ZFP-VEGF increases VEGF mRNA and protein.

<p>(A) VEGF mRNA levels encoding for VEGF₁₂₀, VEGF₁₆₄ and VEGF₁₈₈ isoforms were measured by quantitative real-time PCR at 5 days post-SCI. The bar graph illustrates that administration of ZFP-VEGF resulted in an increase of VEGF mRNA compared with AdV-eGFP and SCI injured control groups. Relative mRNA levels are expressed as the mean ± SEM, n = 4/sham and injured control groups, n = 5/AdV-eGFP and AdV-ZFP-VEGF groups. One-way ANOVA (Holm-Sidak post-hoc) was completed individually for each isoform **p<0.001, *p<0.01. (B) Western blot showing administration of AdV-ZFP-VEGF resulted in increased VEGF-A protein levels at 10 days post-SCI, and (C) Quantification shows a significant increase in VEGF-A 42 kD protein in AdV-ZFP-VEGF treated animals compared with control groups. Optical density (OD) of VEGF-A was normalized to actin. Data are presented as mean ± SEM, n = 4/sham, injured control and AdV-eGFP treated groups and n = 5/AdV-ZFP-VEGF treated group. One-way ANOVA (Holm-Sidak post-hoc) **p<0.02, *p<0.05.