Expression of p21, p27 and p57 in TSCs and TGCs.

<p>Cells were stained for the indicated protein, or for DNA, or for incorporation of EdU (S-phase cells) as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0097434#pone-0097434-g001" target="_blank">Figures 1</a>–<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0097434#pone-0097434-g004" target="_blank">4</a>. M-phase cells were identified by the presence either of condensed chromatids or of mitotic figures. TGCs were examined three (3) days after FGF4 deprivation of TSCs. A minimum of 100 cells was scored in each case in order to determine the fraction of cells undergoing the indicated event. %G phase cells = 100– (%S phase cells+%M phase cells).