The p27 and p57 proteins are localized to the nucleus in G-phase TGCs.

<p>After three days of FGF4 deprivation, wild-type TGCs were cultured for 20 min in the presence of EdU (10 µM) to label S-phase cells. After fixation, EdU was detected using Click-iT chemistry (green), and the cells were stained with either anti-p57 (cyan) (A, C) or anti-p27 (red) (B, C) antibodies, and with Hoechst 33342 to visualize nuclear DNA (gray). Images were acquired with a confocal microscope (63x objective). The bottom panels are higher magnifications of merged images in order to visualize differential staining of EdU, p27 and p57. Scale bars represent 50 µm.