CHD5 is enriched in pericentromeric chromatin of round spermatids.

<p>(A, B) Spread nuclei of spermatids immunostained for CHD5 and counterstained with DAPI. An example of a round spermatid nucleus that is positive for CHD5 expression and displays the high intensity focus in the chromocenter is shown in A and such an example is also indicated with a white arrow in B, the yellow arrow indicates a spermatid nucleus that expresses CHD5, but does not have the extra CHD5 focus. The green arrow indicates a CHD5-negative round spermatid nucleus. (C) Spread nuclei of spermatids immunostained for CHD5 and H3K9me2, and counterstained with DAPI. The nuclear area encompassing the chromatin of the sex chromosome is indicated with a dashed line H3K9me2 marks the chromocenter and the sex chromosome in the left (early) round spermatid nucleus, and mainly the sex chromosome in the right (late) round spermatid nucleus. CHD5 level is very low in the left nucleus, but clearly enriched in the chromocenter and the focus (indicated with a white arrow) adjacent to the chromocenter and Y chromosome chromatin in the right nucleus.