Immunohistochemical localization of AAT in liver and kidney.

<p>Panels A and B depict sections from a normal mouse liver and kidney (A, B, respectively) that were stained with an isotype IgG control antibody (1∶250 dilution), serving as negative controls. Panels C and D depict normal liver (C) and normal kidney (D) probed with 1∶250 dilution of anti- mouse AAT. Mild AAT staining was observed, compared to the isotype negative controls. Panel E depicts a liver section from a mouse with glycerol- induced AKI and probed with anti- AAT. No increase in AAT staining is apparent, compared to normal liver (C). Panel F depicts renal cortical AAT staining 18 hrs post induction of glycerol AKI. Marked staining of renal cortical proximal tubule casts and modest cytoplasmic AAT staining within proximal tubules are apparent. The scale bar = 100 microns.