<p>Huh7.5.1 cells were transfected with full-length RNAs of JFH-1/wt and JFH-1/T416N. Culture medium of each strain was collected and analyzed by 10%–40% of iodixinol density gradient. Fractions were collected, and HCV core Ag and infectivity titers JFH-1/wt (A) JFH-1/T416N (B) were measured.</p
Supernatants harvested from Huh7.5 cells 48 hours post-electroporation of NS4A WT, NS4A Y45F, or ΔE1...
<p><b>(A)</b> Intracellular replication and virus production. Left panel: Replication of HCV RNAs in...
<p>(A and B) Huh7 cells were respectively transfected with JFH1 and 420Bla RNAs and cultured for add...
<p>Huh7.5.1 cells were transfected with full-length RNAs of JFH-1/2G and JFH-1/6B. Culture medium of...
<p>(A) Approximately 22 ml of filtered cell culture fluid harvested 24 h after transfection of Huh-7...
<p>Huh7.5 cells were electroporated with <i>in vitro</i> transcripts of WT or the indicated virus mu...
<p>Physical properties of the nascent virus particles produced in cells stimulated or not with GW396...
<p>The indicated viruses were resolved using an iodixanol step gradient. For each fraction HCV infec...
<p>A) Western blot following the membrane flotation assay in an iodixanol gradient. Briefly, cytopla...
<p>HuH-7 (<b>A</b>), Hep3B (<b>B</b>), HepG2-CD81 (<b>C</b>) and PLC/PRF/5 (<b>D</b>) were electropo...
<p>(A) Long-term culture of JFH-1-transfected cells. The full-genome JFH-1 RNA was transfected into ...
<p>HLMF were inoculated with JFH1-HCVcc 24 hours after plating. Parameters of HCV infection were mon...
<p>HCV genomic RNA transcripts were transfected and compared to J6/JFH1. The J6/JFH1-GND control rem...
<p>A) Huh7.5.1 cells were infected for 24 hours with JFH-1 and then resting pDC-GEN2.2 cells were ad...
<p>(Panel A) JFH1-AM120 RNA was electroporated into Huh-7.5 cells to produce the recombinant adapted...
Supernatants harvested from Huh7.5 cells 48 hours post-electroporation of NS4A WT, NS4A Y45F, or ΔE1...
<p><b>(A)</b> Intracellular replication and virus production. Left panel: Replication of HCV RNAs in...
<p>(A and B) Huh7 cells were respectively transfected with JFH1 and 420Bla RNAs and cultured for add...
<p>Huh7.5.1 cells were transfected with full-length RNAs of JFH-1/2G and JFH-1/6B. Culture medium of...
<p>(A) Approximately 22 ml of filtered cell culture fluid harvested 24 h after transfection of Huh-7...
<p>Huh7.5 cells were electroporated with <i>in vitro</i> transcripts of WT or the indicated virus mu...
<p>Physical properties of the nascent virus particles produced in cells stimulated or not with GW396...
<p>The indicated viruses were resolved using an iodixanol step gradient. For each fraction HCV infec...
<p>A) Western blot following the membrane flotation assay in an iodixanol gradient. Briefly, cytopla...
<p>HuH-7 (<b>A</b>), Hep3B (<b>B</b>), HepG2-CD81 (<b>C</b>) and PLC/PRF/5 (<b>D</b>) were electropo...
<p>(A) Long-term culture of JFH-1-transfected cells. The full-genome JFH-1 RNA was transfected into ...
<p>HLMF were inoculated with JFH1-HCVcc 24 hours after plating. Parameters of HCV infection were mon...
<p>HCV genomic RNA transcripts were transfected and compared to J6/JFH1. The J6/JFH1-GND control rem...
<p>A) Huh7.5.1 cells were infected for 24 hours with JFH-1 and then resting pDC-GEN2.2 cells were ad...
<p>(Panel A) JFH1-AM120 RNA was electroporated into Huh-7.5 cells to produce the recombinant adapted...
Supernatants harvested from Huh7.5 cells 48 hours post-electroporation of NS4A WT, NS4A Y45F, or ΔE1...
<p><b>(A)</b> Intracellular replication and virus production. Left panel: Replication of HCV RNAs in...
<p>(A and B) Huh7 cells were respectively transfected with JFH1 and 420Bla RNAs and cultured for add...