The pattern of SME gene transcripts in breast cancer cells is largely independent of cyclin D1 levels.

<p>For panels (<b>A</b>) through (<b>C</b>), MDA-MB-468 breast epithelial cells were stably-transfected with a sequence encoding cyclin D1. (<b>A</b>) AKR1C1, AKR1C2, and AKR1C3 transcript levels in parental MDA-MB-468 cells (468) and in 3 stably-transfected cell lines (468-cyclin D1-1 through 468-cyclin D1-3) were detected and quantitated by qRT-PCR. (<b>B</b>) CYP19 and 17β-HSD2 transcript levels were similarly quantitated in the 4 cell lines. (<b>C</b>) The cyclin D1 protein levels in parental MDA-MB-468 cells and the 3 cyclin D1-transfectant cell lines analyzed in panels (<b>A</b>) and (<b>B</b>) were detected by immunoblot analysis, with β-actin levels as the loading control. For panels (<b>D</b>) and (<b>E</b>), MCF-7 breast epithelial cells were stably-transfected with a sequence encoding cyclin D1. (<b>D</b>) AKR1C1 and AKR1C3 transcript levels in parental MCF-7 cells and in 3 stably-transfected cell lines (MCF-7-cyclin D1-1 through MCF-7-cyclin D1–3) were detected and quantitated by qRT-PCR. (<b>E</b>) The cyclin D1 protein levels in parental MCF-7 and the 3 cyclin D1-transfectant cell lines analyzed in panel (<b>D</b>) were detected by immunoblot analysis, with β-actin levels as the loading control. For panels (<b>A</b>), (<b>B</b>), and (<b>D</b>), the data are expressed as the mean ± SEM, n = 3 times/group; *<i>p</i><0.05 and **<i>p</i><0.01. Note the differences in Y-axis scales on the graphs for panels (<b>A</b>), (<b>B</b>), and (<b>D</b>).