Effect of stretch on miRNA expression profile in cultured human aortic smooth muscle cells (HASMCs).

<p>(A) HASMCs were exposed to mechanical stretch (16% elongation, 1 Hz) or maintained under static conditions for 12 hr. MiRarray assay was performed to investigate the stretch-altered expression of miRNAs. P<0.05, vs static, n = 3. (B, C, D) Cultured HASMCs were subjected to 16% stretch for 12 hr or maintained under static conditions, Quantitative RT-PCR (qRT-PCR) analysis of mRNA expression of miR-221, miR-145 and miR-21 under stretch. Data are mean±SEM of 4 experiments. *p<0.05, **p<0.01, vs static.