p53 participates in FBI-1 modulating ETS-1 activity.

<p>(A–D) Lovo cells were transfected with FBI-1 expression vector or empty vector. (A–B) Lovo cells were co-transfected with MMP1-Luc vector, p53 siRNA, or control siRNA, p53 vector or empty vector as indicated. Following transfection, cells were harvested for the luciferase assay. The values are the mean ± SE of three independent experiments with similar results. (C–D) Lovo cells were co-transfected with p53 vector or empty vector, p53 siRNA or control siRNA. The ChIP assays were performed with IgG of anti-ETS-1 antibody. (<b>E–F</b>) The luciferase activities changing of EBS-luc, p21-luc and ARE-luc upon FBI-1 and p53 overexpression and knocking down. The values are the mean ± SE of three independent experiments with similar results. *P<0.05 versus the empty vector or the FBI-1 vector (A–D), *P<0.05 versus the empty vector or the p53 vector (A, C), *P<0.05 versus the p53 siRNA vector or the control siRNA vector (B, D).