Expression of <i>dlx5a</i> during zebrafish pectoral development.

<p>Whole mount <i>in situ</i> hybridization for <i>dlx5a</i> in the pectoral region of zebrafish embryos from 24 hpf to 72 hpf. During pectoral fin formation, <i>dlx5a</i> is expressed in apical ectodermal cells of the presumptive pectoral fin bud (PPF) at 24 hpf (A) and in the apical ectodermal ridge (AER) of the early pectoral fin bud (PFB) at 36 hpf (B). At 48 hpf, <i>dlx5a</i> expression is detected in the pectoral fin fold (PFF) and weak expression is observed in endochondral cells (end) of the PFB. Moreover, the transcripts are detected in the developing cleithrum (cl) from 36 hpf to 72 hpf (B–D). The dashed lines in B–C indicate the limit between the AER/PFF structures and cleithrum precursor cells. Note the absence of <i>dlx5a</i> expression in the PFF at 72 hpf (D). Scale bars 50 µm.