Inhibition of PKA activity in cultured cerebellar astrocytes increases glycogen accumulation.

<p>(<b>A</b>) PKA activity in cerebellar astrocytes treated with different concentrations of H89 for 24 hr [mean ±SD, n = 5; ^*<i>P</i><0.05 compared with DMSO (vehicle) treated astrocytes]. (<b>B</b>) Representative immunoblot of p-PKA substrate in astrocytes treated with either DMSO or H89 for 24 hr. Phosphorylation level was normalized against β-Actin protein, the analysis is shown in the lower panel (n = 5; ^*<i>P</i><0.05). Glycogen levels in astrocytes treated with H89 (<b>C</b>) and Rp-8-Cl-cAMPs (<b>D</b>) (mean ±SD, n = 5; ^*<i>P</i><0.05). (<b>E</b>) Immunoblot of pGP in H89 treated astrocytes. Quantification, shown in the lower panel, was performed using total GP protein. Data are represented as mean ±SD, n = 5; ^*<i>P</i><0.05. (<b>F</b>) GP activity in H89 treated astrocytes. Data are mean ±SD, n = 5; ^*<i>P</i><0.05 vs DMSO treated astrocytes. (^*<i>P</i> = Student's t-test).