3V-dependent ATP kinetics of <i>P. falciparum</i> infected RBCs.

<p>A, C. Time course of ATPe concentration (pmol/10⁶ cells) for r-RBCs (ring-infected erythrocytes), t-RBCs (trophozoite-infected erythrocytes) and s-RBCs (schizont-infected erythrocytes) at low parasitemia (<5%, A) and high parasitemia (5–12.5%, C). In the time indicated by the arrow, cells were exposed to “3V”, a cAMP activating cocktail containing 10 mM isoproterenol, 30 mM forskolin and 100 mM papaverine. For a comparison, similar experiments with h-RBCs are shown. B, D. 3V-dependent increases of [ATPe] calculated from A and C. Values are expressed as ΔATP₁ for low parasitemia (B) and high parasitemia (D). Results are means ± SEM. (*p<0.05, ***p<0.001). (N, n), with N = independent preparations, n = replicates. E. Initial rate of [ATPe] decay (pmol/10⁶ cells/min) taken from data of C.