Analysis of quality and integrity of extracted nucleic acids.

<p>(<b>A</b>) Genomic DNA and total RNA (∼0.5 µg) isolated simultaneously from four strains of <i>E. siliculosus</i> (RHO12; LIA4A; REP10–11; EC524), using initial biomass of 25, 50 and 100 mg (gel stained with ethidium bromide). DNA shows an intact single band whilst RNA shows the clear cytosolic and plastid (Cp) ribosomal bands. (<b>B</b>) Genomic DNA contamination is effectively removed by DNase treatment, whilst the pure RNA retains intactness and quality. RNA species of low molecular weight are also apparent. M: RNA Ladder, High Range (Fermentas, Italy).