Delta24-RGD treatment results in long-term survival in the GL261 immune competent model.

<p>A) Kaplan-Meier survival plot of C57BL/6 mice injected with GL261 cells and injected five days later with Delta24-RGD (n = 8, red line), PBS (n = 8, blue line) or received dexamethasone treatment (daily 7.5 mg/kg, upon day 55 (arrow) (n = 8, light purple line) and Delta24-RGD+dexamethasone (n = 8, green line). Non-tumor bearing mice received dexamethasone as a control for dexamethasone toxicity (n = 4, black line). B) To test the effect of dexamethasone on viral efficacy in vitro GL261 cells were infected in triplicate with Delta24-RGD in the absence (light grey bar) and presence of 0.2, 2, 20 and 200 µM dexamethasone (0.2 d to 200 d). The mean viability is expressed as percentage of the control ± SD (white bar). C) In vivo staining for the adenoviral protein hexon shows a similar staining pattern with dexamethasone (10 mg/kg/day) compared to the control (shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0097495#pone-0097495-g001" target="_blank">figure 1C</a>). Representative images of two mice per time-point are shown.