Expression of CCL20 and CCR6 in colon cancer.

<p>(A) CCL20 concentration was assayed by ELISA in 12 matched samples of colon cancer and adjacent normal colon tissue after homogenizing approximately 100 mg of tissue into 100 µl PBS. The Wilcoxon signed-rank test was used to assess significance. (B) Representative photomicrograph of a section of paraffin-embedded human colon cancer tumor tissue immunohistochemically stained for CCL20. The regions in the squares are shown magnified. (C) Relative expression of human <i>Ccr6</i> is compared in 10 matched samples of colon cancer and adjacent uninvolved colon tissue as measured by semiquantitative RT-PCR. (D) Representative photomicrograph of a section of paraffin-embedded human colon cancer tumor tissue immunohistochemically stained for CCR6. The regions in the squares are shown magnified.