Comparison of endogenous ABA contents in imbibed seeds of different transgenic lines.

<p>ABA analysis was carried out using the radioimmumoassay (RIA) method. Thirty imbibed rice seeds were homogenized in 1 ml of distilled water and then shaken at 4°C overnight. The homogenates were centrifuged at 12,000 g for 10 min at 4°C and the supernatant were used directly for ABA assay. Values shown are means of three biological replications±SE. Asterisks (*) indicate significant difference (<i>P</i><0.05) between the endogenous ABA contents of transgenic lines compared with the wildtype.