Truncation of the gHcyt reduces viral titers in melanoma cells.

<p>(A) Replication kinetics of pOka-gH[834StopV5], pOka-gH[Δ834-841], pOka-gH[TL], and pOka in melanoma cells over six days. Individual points represent the mean of harvested infected cells titrated in triplicate, including the titrated inoculum at Day 0. Titers were measured in log₁₀ of plaque forming units per milliliter (PFU/mL). The error bars represent the SEM. Statistical differences between pOka-gH[TL] and the other viruses were evaluated by two-way ANOVA (*P<0.05, **P<0.01, ***P<0.001). (B) Replication kinetics of pOka-gH[Y835A], pOka-gH[Y835F], pOka-gH[V5], and pOka in melanoma cells over six days with no statistical differences were observed between pOka and the mutant viruses. (C and D) Box and whisker plots of plaque sizes measured (n = 30) in melanoma cells infected with (C) pOka-gH[834StopV5], pOka-gH[Δ834-841], pOka-gH[TL], and pOka or (D) pOka-gH[Y835A], pOka-gH[Y835F], pOka-gH[V5], and pOka at four days post infection. Area of plaques was measured in millimeter squared (mm²). The boxes represent the 10–90% percentile with the median indicated by the band and the outliers (<10% or >90% percentile) represented as individual black dots.