Pathogenesis of VZV with mutations in the gHcyt in human skin xenografts.

<p>Confocal micrographs of 5 µm sections of human skin xenografts with the highest titers infected with either pOka or pOka-gH[Δ834-841], -gH[Y835A], -gH[Y835F], -gH[TL], and -gH[V5] at 21 dpi. The sections were stained for capsids (ORF23; green), gE (red), TGN46 (violet), and nuclei (Hoechst 33342; blue). The scale bar for skin implants infected with pOka (top left) and gH[Δ834-841] (top right) represent 200 µm. White squares are shown in higher magnification immediately below (A = pOka, B = Δ834-841). Higher magnification images consist of a four-color merge (top), two color-ORF23 and gE merge (bottom left), and two color-TGN46 and nuclei merge (bottom right). Lesions were not detected for pOka-gH[TL]-infected xenografts. The scale bars for higher magnification images represent 50 µm.