Amino acids 834-841 of the gHcyt are dispensable for intracellular trafficking and endocytosis of gH during infection of melanoma cells.

<p>Confocal microscopy of melanoma cells at 24(Uninfected) or infection with pOka-gH[Δ834-841], pOka-gH[TL], pOka-gH[Y835A], pOka-gH[Y835F], pOka-gH[V5], pOka-gH[834StopV5], or pOka viruses. Cells were stained for gH (red), early endosome antigen (EEA1; green), trans-Golgi network (TGN46; green) and nuclei (Hoechst 33342; blue). White arrows indicate colocalization of EEA1 and gH, which highlight representative endocytic vesicles containing gH. The scale bars represent 20 µm.