Viral production from latently infected cells <i>ex vivo</i>.

<p>Panel A. HIV RNA copies/ml measured in the supernatant of resting CD4+ T cells isolated from treated HIV+ individuals (S: cells from single individuals, P: pooled cells from 2-3 individuals) and stimulated <i>ex vivo</i> with DMSO (control, black), SAHA (blue) or TCR (green) for 1, 2 or 4 days. Panel B. Box plot showing HIV RNA copies/ml measured in the supernatant of resting CD4+ T cells isolated from treated HIV+ individuals and stimulated <i>ex vivo</i> with DMSO (control, black), SAHA (blue) or TCR (green) for 1, 2 or 4 days. Each circle represents a different cellular sample. Bars: min to max. Solid line: median. Panel C. Immunoblot of histones (1 µg) extracted from stimulated <i>ex vivo</i> cells isolated from treated HIV+ individuals. H3K27Ac: anti-histone 3, acetylated Lysine 27; H3K9Ac: anti-histone 3, acetylated Lysine 9; H3: anti-histone 3 (total).