MLC1 overexpression, but not BSP1, suppresses <i>rvs167Δ iqg1-1</i> synthetic lethality.

<p>Indicated strains were grown to exponential phase in glucose containing media, washed into galatose/raffinose media and then spotted onto indicated media and grown at temperature shown before imaging. MLC1 overexpression suppresses <i>rvs167Δ iqg1-1</i> synthetic lethality (<b>A</b>) whereas BSP1 overexpression does not (<b>B</b>). Endocytosis is required for BSP1-mediated suppression of <i>iqg1-1</i> at the restrictive temperature (<b>C</b>). <b>D</b> BSP1 localisation unaltered in amphiphysin null cells. BSP1-GFP and eCFP-TUB3 represent maximum and average intensity projections respectively. 5 representative examples of BSP1-GFP rings at the bud neck in the z/x plane (i. e. looking through the division site) are shown. Scale bar = 2.5 µm. (E) Chs3-GFP polarity altered in <i>rvs</i> mutant cells. Fluorescent images are maximum intensity projections. Image reconstructions of bud neck demonstrate Chs3-GFP localizes to rings and septa. Scale bar = 2.5 µm.