Immunofluorescence analysis of vimentin, CD31, and IL-10Rα expression in HS and NS tissue.

<p>(a) Immunofluorescence staining showed that vimentin (green, FITC-conjugated secondary antibody) was localized in HS (upper row) and NS tissue (lower row), with an abundance of vimentin-positive fibroblasts in the HS dermis. Scale bar, 100 µm. (b) Vimentin (green, FITC-conjugated secondary antibody) and IL-10Rα (red, Cy3-conjugated secondary antibody) immunofluorescence staining showed co-localization of both proteins in HSFs (upper row) and NSFs (lower row). Scale bar, 100 µm. (c) CD31 (red, Cy3-conjugated secondary antibody) and IL-10Rα (green, FITC-conjugated secondary antibody) were co-localized on vascular endothelial cells in HS (upper row) and NS tissue (lower row). Scale bar, 200 µm.